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Although gillnet fisheries are increasingly common in Korea, few studies have conducted sea trials using tie-down gillnets. Here, we analyzed the mesh size selectivity using tie-down gillnets with four different mesh sizes (84, 90, 105, and 120 mm) to catch blackfin flounders. A total of 10 sea trials were conducted at depths of 100–140 m in the waters of Yangyang County, Gangwon Province, Korea, and the catching efficiency and mesh size selectivity were comparatively analyzed. The net with a mesh size of 84 mm showed the highest number of catches (373), followed by the 90-mm (363) and 105-mm (307) meshes. The results of the master curve estimation showed that the larger the mesh size, the higher the total length representing the same retention probability. Therefore, the catch rate of small fish decreased with the increasing mesh size. For the 84-mm net, the 50% selection length for small fish (total length ≤ 170 mm) was estimated at 186.9 mm, which was approximately 17 mm larger than the length limit. The results show that when using tie-down gillnets, the size of the fish caught changed according to the changes in the mesh size.